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  • 1
    Electronic Resource
    Electronic Resource
    A stable cytoplasmic male-sterile line of Brassica juncea carrying restructured organelle genomes from the somatic hybrid Trachystoma ballii+B. juncea (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A cytoplasmic male-sterile (CMS) line of Brassica juncea has been developed by combining the cytoplasm originating from the somatic hybrid Trachystoma ballii+B. juncea, and the nucleus of B. juncea cv. Pusa Bold by repeated backcrossing. Male-sterile plants closely resembled the normal fertile B. juncea in general morphology, but had delayed flowering (5–7 days) when compared with fertile ‘Pusa Bold’ which flowered in 45 days. Stamens of the male-sterile line were transformed into petaloid structures. Pollen abortion occurred after tetrad formation. Female fertility of the male-sterile line was normal. Molecular analysis of organelle genomes indicated extensive mitochondrial DNA recombinations in the CMS line. Preliminary analysis of the chloroplast genome of the CMS line also indicated chloroplast DNA recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00826.x
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular mapping of a locus controlling resistance to Albugo candida in Indian mustard (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Brassica juncea (L.) Czern & Coss is widely grown as an oilseed crop in the Indian subcontinent. White rust disease caused by Albugo candida (Pers.) Kuntze is a serious disease of this crop causing considerable yield loss every year. The present study was undertaken to identify molecular markers for the locus controlling white rust resistance in a mustard accession, BEC-144, using a set of 94 recombinant inbred lines (RILs). The screening of individual RILs using an isolate highly virulent on the popular Indian cultivar ‘Varuna’ revealed the presence of a major locus for rust resistance in BEC-144. Based on screening of 186 decamer primers employing bulked segregant analysis (BSA), 11 random amplified polymorphic DNA markers were identified, which distinguished the parental lines and the bulks. Five of these markers showed linkage with the rust resistance locus. Two markers, OPN0l000 and OPB061000, were linked in coupling and repulsion phases at 9.9 cM and 5.5 cM, respectively, on either side of the locus. The presence of only two double recombinants in a population of 94 RILs suggested that the simultaneous use of both markers would ensure efficient transfer of the target gene in mustard breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00658.x
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  • 3
    Electronic Resource
    Electronic Resource
    Shoot regeneration in stem expiants and its amenability to Agrobacterium tumefaciens mediated gene transfer in Brassica carinata (1992)
    Springer
    Plant cell reports 11 (1992), S. 359-362 
    Details
    ISSN: 1432-203X
    Keywords: Brassica carinata ; Agrobacterium tumefaciens ; Shoot regeneration ; Gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immature stem segments of seven different genotypes of Brassica carinata produced shoots with variable frequencies when cultured in MS medium with BAP and picloram at 0.2 mg/l each. Line 171, which produced shoots with 100% efficiency from both cut ends of the expiant, was selected for testing the amenability of this regeneration protocol for genetic transformation. A non-oncogenic Agrobacterium tumefaciens containing plasmid PCV 730, a binary vector carrying resistance genes for kanamycin and hygromycin, was used. A cocultivation period of 4 d with a bacterial concentration of approximately 2.5×10 cells/ml, followed by a recovery period of 2 d, produced transformed shoots that could be selected and rooted in the presence of kanamycin at 15 mg/l. Transformation was confirmed by neomycin phospho-transferase assay and Southern blot analysis. Seed analysis of transformed plants indicated that kanamycin resistance was inherited in the progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233366
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  • 4
    Electronic Resource
    Electronic Resource
    Diplotaxis catholica + Brassica juncea somatic hybrids: molecular and cytogenetic characterization (1995)
    Springer
    Plant cell reports 14 (1995), S. 593-597 
    Details
    ISSN: 1432-203X
    Keywords: Brassica juncea ; Diplotaxis catholica ; Somatic hybrids ; Organelle constitution ; Mitochondrial DNA recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric somatic hybrids Diplotaxis catholica (2n=18) + Brassica juncea (2n=36) were produced by fusing mesophyll protoplasts of the former and hypocotyl protoplasts of the latter using polyethylene glycol. Out of 52 somatic embryos, 24 produced plants of intermediate morphology. Cytological analysis of 16 plants indicated that 15 were symmetric hybrids carrying 54 chromosomes, the sum of the parental chromosome numbers. One hybrid was asymmetric with 45 chromosomes. Nuclear hybridity of five putative hybrids was confirmed by the Southern hybridization pattern of full length 18s-25s wheat nuclear rDNA probe which revealed the presence of Hind III fragments characteristic of both the parental species. The hybridization pattern of mitochondria specific gene probe cox I indicated that three of the hybrids carried B. juncea mitochondria and one carried mitochondria of D. catholica. Presence of novel 3.5 kb Hind III and 4.8 kb Bgl II fragments suggested the occurrence of mtDNA recombination in one of the hybrids. The hybrids were pollen sterile. However, seeds were obtained from most of the hybrids by back crossing with B. juncea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231945
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  • 5
    Electronic Resource
    Electronic Resource
    Random chloroplast segregation and mitochondrial genome recombination in somatic hybrid plants of Diplotaxis catholica+Brassica juncea (1998)
    Springer
    Plant cell reports 17 (1998), S. 814-818 
    Details
    ISSN: 1432-203X
    Keywords: Key words Random chloroplast segregation ; Mitochondrial genome recombination ; Diplotaxis catholica ; Brassica juncea ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Detailed molecular analysis of the somatic hybrid plants of Diplotaxis catholica+B. juncea indicated random chloroplast segregation. One of the five hybrid plants analyzed derived its chloroplasts from D. catholica and two hybrids had chloroplasts of B. juncea origin. Two hybrid plants maintained mixed population of chloroplasts. The mitochondrial (mt) genomes of the fusion partners had undergone recombinations. Occurrence of fragments specific to both the parents in HindIII digestion followed by atp 9 probing, as in hybrid DJ5, provided evidence for intergenomic mitochondrial recombination between D. catholica and B. juncea. Similar mt genome organization in two hybrids (DJ3 and DJ6) suggested that intergenomic recombination may be preferred at specific sites. Hybrid DJ1 had about 70% similarity to D. catholica in mt genome organization. mt genomes of hybrids DJ2, 3, 5, and 6 differed from B. juncea by 14.3–28%. The significance of these novel mt genome organizations in developing novel male sterility systems is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050489
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  • 6
    Electronic Resource
    Electronic Resource
    Approximation of double integrals of analytic functions of two complex variables (1986)
    Springer
    Computing 37 (1986), S. 357-364 
    Details
    ISSN: 1436-5057
    Keywords: (1970) Primary 65D30 ; Analytic function ; double integral ; approximation ; degree of precision
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Es wird eine 9punktige Formel vom Grad 5 zur numerischen Approximation von Doppelintegralen komplexer Variablen samt Fehlerschranke hergeleitet.
    Notes: Abstract A nine-point degree five rule for the numerical approximation of double integrals of analytic functions of complex variables and its error bound have been derived.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02251093
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular mapping and tagging of genes in crop plants (1996)
    Springer
    Genetica 97 (1996), S. 313-320 
    Details
    ISSN: 1573-6857
    Keywords: chickpea ; molecular mapping ; mustard ; quantitative traits ; RAPD ; RFLP ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In India, molecular mapping and tagging of agronomically important genes using RFLP and RAPD markers have been carried out in three different crops: rice, mustard and chickpea. In rice, tagging of genes for resistance to gall midge and blast has been accomplished. Molecular mapping of cooking quality traits in rice is in progress. For fingerpringting rice cultivars, suitable probe enzyme combinations have been identified. In mustard, a partial RFLP linkage map has been constructed and one of the yellow seed-coat colour loci has been mapped. Significant associations of RFLP markers with quantitative traits have also been established. Potential use of RAPD markers to identify heterotic groups among mustard accessions has been demonstrated. In chickpea, the occurrence of considerable interspecific DNA polymorphism as revealed by RAPD analysis has facilitated construction of a partial linkage map.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00055317
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  • 8
    Electronic Resource
    Electronic Resource
    Recombination between chloroplast genomes of Trachystoma ballii and Brassica juncea following protoplast fusion (1998)
    Springer
    Molecular genetics and genomics 260 (1998), S. 357-361 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsTrachystoma ballii ; Brassica juncea ; Somatic hybrid ; Chloroplast genome recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We document here the presence of a recombinant plastome in a cytoplasmic male sterile (CMS) line of Brassica juncea developed from the somatic hybrid Trachystoma ballii + B. juncea. Restriction endonuclease digestion of the chloroplast (cp) DNA has revealed that the recombinant plastome gives rise to novel fragments in addition to the parent-specific fragments. Analysis of the 16S rRNA region by Southern hybridization shows no variation between B. juncea, T. ballii and the CMS line. The rbcL gene region of the recombinant plastome is identical to that in T. ballii. Analysis with probes for psbA and psbD using single and double DNA digests indicates that the hybridization patterns of the recombinant plastome are identical to those of the parents in digests obtained with some restriction enzymes, while novel bands hybridize to probes in other digests. In the psbA region, a B. juncea-specific PstI site and a T. ballii-specific EcoRI site are found in the recombinant plastome. The psbD region of the recombinant plastome contains a B. juncea-specific HindIII site and T. ballii-specific BamHI and HpaII sites. These results indicate the occurrence of intergenomic recombination between the chloroplasts of T. ballii and B. juncea in the somatic hybrid from which the CMS line was developed. The recombined plastome appears to be a mosaic of fragments specific to both parents and the recombination event has occurred in the single-copy regions. These recombinational events have not caused any imbalance in the recombinant plastome in terms of chloroplast-related functions, which have remained stable over generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050904
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  • 9
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    Genomic Constitution and Genetic Relationship among the Tropical and Subtropical Indian Sugarcane Cultivars Revealed by AFLP (2005)
    Wiley
    Details
    Publication Date: 2005-09-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 10
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    AFLP Analysis of the Phenetic Organization and Genetic Diversity in the Sugarcane Complex, Saccharum and Erianthus (2006)
    Springer
    Details
    Publication Date: 2006-02-27
    Print ISSN: 0925-9864
    Electronic ISSN: 1573-5109
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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