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  • Articles  (142)
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  • 1
    Electronic Resource
    Electronic Resource
    Cycling technique for the determination of femtomole amounts of sulfite (1989)
    s.l. : American Chemical Society
    Analytical chemistry 61 (1989), S. 1755-1758 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00190a029
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  • 2
    Electronic Resource
    Electronic Resource
    In memoriam Hermann Remmert (1995)
    Springer
    Naturwissenschaften 82 (1995), S. 1-1 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01167862
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  • 3
    Electronic Resource
    Electronic Resource
    Size-Related Differences in Carbon Isotope Discrimination in the Epiphytic Orchid, Dimerandra emarginata (1999)
    Springer
    Naturwissenschaften 86 (1999), S. 39-40 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001140050566
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  • 4
    Electronic Resource
    Electronic Resource
    Gibberellinaktivität von Siebröhrensäften (1964)
    Springer
    Naturwissenschaften 51 (1964), S. 145-146 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00631168
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  • 5
    Electronic Resource
    Electronic Resource
    δ13C values of CO2 from soil respiration on sites with crops of C3 and C4 type of photosynthesis (1986)
    Springer
    Oecologia 69 (1986), S. 305-308 
    Details
    ISSN: 1432-1939
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Based on the differential discrimination between 13CO2 and 12CO2 during photosynthetic CO2 fixation by C3 and C4 plants, the relative contribution of organic soil material of varying age can be checked by measuring the δ13C value of CO2 evolved during soil respiration. The CO2 from a soil cropped only with C3 plants for at least 7 years showed a δ13C value very close to that of C3 plant tissue. Correspondingly, a soil bearing the C4 plant maize for at least 6 years had a δ13C value for the respiratory CO2 quite near to that of a typical C4 plant. A soil cropped with maize in the year of study only showed intermediate δ13C values, with a tendency towards C4. With soil standardised for cultivation and cropping, a quantification of the part that fresh organic matter and especially the last year plant residues play in soil respiration, can be achieved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00377638
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  • 6
    Electronic Resource
    Electronic Resource
    Über die Zusammensetzung des Zikadenschaumes (1957)
    Springer
    Journal of comparative physiology 40 (1957), S. 549-555 
    Details
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340767
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  • 7
    Electronic Resource
    Electronic Resource
    Quantitative analysis of abscisic acid in needles of Abies alba Mill. by electron capture gas chromatography (1993)
    Springer
    Trees 7 (1993), S. 175-181 
    Details
    ISSN: 1432-2285
    Keywords: Abies alba ; Abscisic acid ; Damage ; Gas chromatography ; Needles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amount of abscisic acid (ABA) in needles of silver fir from a natural location was investigated with regard to position in the crown, damage, seasonal variation, and needle age. Because of problems of quantification of ABA in coniferous needles, which contain numerous secondary plant products, a method for reliable determination of both isomers cis-trans-ABA (c-ABA) and transtrans-ABA (t-ABA) was developed. By means of gas chromatography (GC) using an electron capture detector (BCD) and a programmed temperature vaporizer (PTV) injector complete separation of both compounds was achieved. Two different pairs of fir were investigated — in each case a damaged and a healthy tree. Needles from both trees from the first and the second pair collected in September contained 500–1100 ng c-ABA/g fresh weight (FW), and the concentrations of t-ABA varied from 400 to 700 ng/g FW. Investigations from the second pair show highest amounts of 2900 ng/g Fw c-ABA and 1800 ng/g FW of t-ABA in May and June. For the first pair a higher c-ABA content was found in needles from the top of the crown than in those from the middle and the base. This difference could not be confirmed in the analysis of the second pair. Because of the strong natural deviation no statistically significant difference between the healthy and the damaged tree was found. The first pair of firs examined showed a higher t-ABA concentration than the second one. In this case the highest amount was found in the top of the crown. Methodical mistakes during the clean-up procedure and in quantification by gas chromatography could be excluded. The presence of c- and t-ABA in the purified extract was corroborated by mass spectrometry. With regard to the seasonal variation both isomers of ABA show an unequivocal trend. The maximum concentration is achieved in May to June, whereas the content is minimal in August/September. In any case the level of t-ABA is lower than that of c-ABA. No correlation between the amount of ABA and the needle age could be established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199619
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  • 8
    Electronic Resource
    Electronic Resource
    Pyridine nucleotide levels and activities of dehydrogenases in cambial derivatives ofRobinia pseudoacacia L. (1996)
    Springer
    Trees 10 (1996), S. 325-330 
    Details
    ISSN: 1432-2285
    Keywords: Cambial differentiation ; Glucose-6-phosphate dehydrogenase ; 6-Phosphogluconate dehydrogenase ; NAD kinase ; Pyridine nucleotides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Despite the importance of the vascular cambial differentiation, little is known about its regulation. In order to address this problem we attempted to biochemically characterize differentiating xylem and phloem elements during the early stages of development. By applying techniques of quantitative histochemistry we show that the total pool size of pyridine nucleotides is similar in the phloem (PD) and xylem (XD) oriented derivatives of the cambial zone of trees ofRobinia pseudoacacia L. Within the PD zone, the amount of NAD + NADH exceeded that of NADP + NADPH [around 600 versus 200 pmol (mg dry weight)-1], possibly indicative of a preponderance of catabolic pathways (ratio of NADH∶NAD about 1). In contrast, the NADP(H) system dominated in the XD zone. This coincided with a high activity of NAD kinase. In addition, the extractable activities of the key enzymes of the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, were greatly increased. At a ratio of NADPH∶NADP of approximately 1, this could be indicative of increased rates of reductive biosyntheses, and could thus well be involved in early steps of the formation of phenols and lignin monomers. Taken together, this first approach clearly shows that phloem-oriented and xylem-oriented cambial descendents exhibit distinct differences in their biochemical patterns even in early stages of differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02340779
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  • 9
    Electronic Resource
    Electronic Resource
    Lipids and lipolytic enzymes in the trunkwood ofRobinia pseudoacacia L. during heartwood formation (1996)
    Springer
    Trees 10 (1996), S. 366-375 
    Details
    ISSN: 1432-2285
    Keywords: Acyllipids ; Heartwood formation ; Phospholipids ; Robinia pseudoacacia L. ; Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The radial distribution of membrane and storage lipids was determined in the trunkwood ofRobinia pseudoacacia L. The trees were felled in November at the time of heartwood formation and fluctuations in the amount and composition of phospholipids, free sterols, steryl esters, diand triacylglycerols, and free fatty acids were investigated across the sapwood-heartwood boundary. The individual compounds were identified and quantified by thin layer chromatography, enzymatic and colorimetric assays, and by capillary gas chromatography. Phospholipids show a significant decrease towards the boundary area, and in the heartwood only trace amounts can be detected. The same pattern is observed for free sterols in the sapwood; in the heartwood, however, they reach maximum values with increasing depth of the trunk. Steryl esters exhibit a complementary behaviour by accumulating at the periphery of the heartwood. No concentration changes are found in the total amounts of diacylglycerols and free fatty acids. In contrast, the triacylglycerol concentration declines steadily across the trunk. With regard to qualitative composition, free fatty acids and the fatty acid moieties of the esterified constituents vary in their chain length from 14 to 24 carbon atoms and have up to three double bonds. A radial gradient in the ratio saturated/unsaturated fatty acids can be observed: except for the phospholipid fraction the relative amounts of unsaturated fatty acids increase in centripetal direction. Seven phospholipids were identified: phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, and phosphatidic acid, which constitutes the major proportion. In the sterol group, sitosterol is the most abundant component. The composition of the esterified sterols remains constant across the trunk cross-section, whereas the relative frequencies of individual free sterols change markedly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02185640
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  • 10
    Electronic Resource
    Electronic Resource
    Lipids and lipolytic enzymes in the trunkwood ofRobinia pseudoacacia L. during heartwood formation (1996)
    Springer
    Trees 10 (1996), S. 376-381 
    Details
    ISSN: 1432-2285
    Keywords: Acylesterhydrolase ; Heartwood formation ; Lipase ; Robinia pseudoacacia L. ; Phospholipases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The radial profile of lipase and phospholipase activities was determined in the trunkwood ofRobinia pseudoacacia L. The trees were felled in November at the time of heartwood formation and alterations in the enzymatic activities were investigated across the sapwood and heartwood. Methods employed include gaschromatographic, colorimetric and enzymatic assays. On a dry weight basis, the hydrolysis of the artificial substrate pnitrophenylpalmitate shows a maximum activity in growth ring 4; however, the assay has proved not to be specific for lipase. In contrast, lipase analyses (triacylglycerol acylhydrolase; E.C. 3.1.1.3) with an authentic substrate show activity peaks in growth rings 1 and 4. With protein as a reference the highest activity is found in growth ring 5. A similar tendency is observed for phospholipase A1 (E.C. 3.1.1.32) and phospholipase A2 (E.C. 3.1.1.4). Phospholipase C (E.C. 3.1.4.3) activity decreases towards the sapwood-heartwood boundary; negligible traces of activity are detected in the heartwood, whereas, based on the protein content, growth ring 4 yields maximal activity. Phospholipase D (3.1.4.4) exhibits the same radial pattern with regard to protein content as a reference. On a dry weight basis there is a significant increase within the sapwood area, while in the heartwood the activity drastically decreases. The enzyme activities are discussed in relation to degradative processes within the plasma membranes and the hydrolysis of reserve lipids during heartwood formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02185641
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