ISSN:
1432-203X
Keywords:
Key wordsCoffea arabica
;
In vitro selection Colletotrichum kahawae
;
Phytotoxin
;
Coffee berry disease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Hypocotyl-derived calli of genotypes and segregating populations of Coffea arabica, differing in susceptibility to Colletotrichum kahawae, were used to produce cell suspensions and protoplasts which were exposed to partially purified culture filtrates (PPCFs) prepared from the pathogen. The growth and viability of PPCF-treated cells and protoplasts were measured using packed cell volume, fluorescein diacetate staining and a colorimetric assay involving the tetrazolium salt MTT. Differential responses of cells and protoplasts were influenced by genotype, time of exposure and PPCF concentration. Protoplasts of resistant genotypes responded differentially from more susceptible genotypes as early as 4 h after challenge with the phytotoxin, suggesting that they were more sensitive than cell suspensions to the treatments. Protoplasts exposed to PPCFs from C. kahawae may therefore be used to screen and select genotypes resistant to, or tolerant of, coffee berry disease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050316
Permalink
