Publication Date:
2013-10-19
Description:
We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lajoie, Marc J -- Rovner, Alexis J -- Goodman, Daniel B -- Aerni, Hans-Rudolf -- Haimovich, Adrian D -- Kuznetsov, Gleb -- Mercer, Jaron A -- Wang, Harris H -- Carr, Peter A -- Mosberg, Joshua A -- Rohland, Nadin -- Schultz, Peter G -- Jacobson, Joseph M -- Rinehart, Jesse -- Church, George M -- Isaacs, Farren J -- 1DP5OD009172-01/OD/NIH HHS/ -- DP5 OD009172/OD/NIH HHS/ -- K01 DK089006/DK/NIDDK NIH HHS/ -- K01DK089006/DK/NIDDK NIH HHS/ -- T32 GM007205/GM/NIGMS NIH HHS/ -- T32GM07205/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2013 Oct 18;342(6156):357-60. doi: 10.1126/science.1241459.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24136966" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Substitution/genetics
;
Amino Acids/*genetics
;
Bacteriophage T7/*physiology
;
Codon, Terminator/*genetics
;
Escherichia coli/*genetics/*virology
;
Escherichia coli Proteins/genetics
;
Genetic Engineering
;
Genome, Bacterial
;
Organisms, Genetically Modified/*genetics/*virology
;
Peptide Chain Termination, Translational/genetics
;
Peptide Termination Factors/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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