Publication Date:
1993-07-30
Description:
Models of DNA replication in Escherichia coli involve an asymmetric DNA polymerase complex that replicates concurrently the leading and the lagging strands of double-stranded DNA. The effect of asymmetry on mutagenesis was tested with pairs of plasmids containing the unidirectional ColE1 origin of replication and a single lesion located in the leading or lagging strand. The lesion used was the covalent adduct that the chemical carcinogen N-2-acetylaminofluorene (AAF) forms with the C-8 position of guanine. Whether SOS was induced or not, mutations arose at about a 20-fold higher frequency when the AAF adduct was located in the lagging strand than when in the leading strand.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Veaute, X -- Fuchs, R P -- New York, N.Y. -- Science. 1993 Jul 30;261(5121):598-600.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Unite Propre de Recherche Cancerogenese et Mutagenese Moleculaire et Structurale, Institut de Biologie Moleculaire et Cellulaire Centre National de la Recherche Scientifique, Strasbourg, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8342022" target="_blank"〉PubMed〈/a〉
Keywords:
2-Acetylaminofluorene/metabolism/toxicity
;
Base Sequence
;
DNA Damage
;
DNA Polymerase I/metabolism
;
DNA Polymerase III/metabolism
;
*DNA Replication
;
DNA, Bacterial/biosynthesis/*genetics
;
Escherichia coli/*genetics/metabolism
;
*Frameshift Mutation
;
Guanine/metabolism
;
Molecular Sequence Data
;
*Mutagenesis
;
Plasmids
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
