Publication Date:
1989-05-05
Description:
Tumor promoters may bring about events that lead to neoplastic transformation by inducing specific promotion-relevant effector genes. Functional activation of the transacting transcription factor AP-1 by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) may play an essential role in this process. Clonal genetic variants of mouse epidermal JB6 cells that are genetically susceptible (P+) or resistant (P-) to promotion of transformation by TPA were transfected with 3XTRE-CAT, a construct that has AP-1 cis-enhancer sequences attached to a reporter gene encoding chloramphenicol acetyltransferase (CAT). Transfected JB6 P+, but not P- variants, showed TPA-inducible CAT synthesis. Epidermal growth factor, another transformation promoter in JB6 cells, also caused P+ specific induction of CAT gene expression. These results demonstrate an association between induced AP-1 function and sensitivity to promotion of neoplastic transformation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bernstein, L R -- Colburn, N H -- New York, N.Y. -- Science. 1989 May 5;244(4904):566-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Johns Hopkins University, Department of Biology, Baltimore, MD 21218.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2541502" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line
;
*Cell Transformation, Neoplastic
;
Chloramphenicol O-Acetyltransferase/genetics
;
Cloning, Molecular
;
DNA-Binding Proteins/genetics/*physiology
;
Epidermal Growth Factor/pharmacology
;
Epidermis
;
Gene Expression Regulation
;
Genetic Variation
;
Kinetics
;
Mice
;
Nucleic Acid Hybridization
;
Plasmids
;
Promoter Regions, Genetic
;
Proto-Oncogene Proteins
;
Proto-Oncogene Proteins c-jun
;
Simplexvirus/genetics
;
Tetradecanoylphorbol Acetate/*pharmacology
;
Transcription Factors/genetics/*physiology
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
