Publication Date:
1990-04-27
Description:
The nitrogen regulatory protein NtrC of enteric bacteria activates transcription of the glnA gene by catalyzing isomerization of closed complexes between RNA polymerase and the glnA promoter to open complexes. NtrC binds to sites upstream of glnA that have properties of eukaryotic transcriptional enhancers. NtrC-binding sites were found to facilitate open complex formation when these sites and the glnA promoter were located on different rings of a singly linked catenane, but not when the two rings were decatenated. The results provide evidence that NtrC contacts RNA polymerase-promoter complexes in a process mediated by formation of a DNA loop. NtrC-binding sites serve to tether NtrC near the glnA promoter, thereby increasing the frequency of collisions between NtrC and polymerase-promoter complexes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wedel, A -- Weiss, D S -- Popham, D -- Droge, P -- Kustu, S -- GM 38361/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1990 Apr 27;248(4954):486-90.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Plant Pathology, University of California, Berkeley 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1970441" target="_blank"〉PubMed〈/a〉
Keywords:
Bacterial Proteins/*metabolism
;
Binding Sites
;
DNA Transposable Elements
;
DNA, Bacterial/metabolism
;
DNA, Superhelical/metabolism
;
DNA-Binding Proteins/*metabolism
;
DNA-Directed RNA Polymerases/metabolism
;
*Enhancer Elements, Genetic
;
Glutamate-Ammonia Ligase/*genetics
;
Nucleotidyltransferases/metabolism
;
PII Nitrogen Regulatory Proteins
;
Plasmids
;
*Promoter Regions, Genetic
;
Templates, Genetic
;
*Trans-Activators
;
*Transcription Factors
;
*Transcription, Genetic
;
Transposases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
