Publication Date:
1999-07-27
Description:
Candida glabrata is an important fungal pathogen of humans that is responsible for about 15 percent of mucosal and systemic candidiasis. Candida glabrata adhered avidly to human epithelial cells in culture. By means of a genetic approach and a strategy allowing parallel screening of mutants, it was possible to clone a lectin from a Candida species. Deletion of this adhesin reduced adherence of C. glabrata to human epithelial cells by 95 percent. The adhesin, encoded by the EPA1 gene, is likely a glucan-cross-linked cell-wall protein and binds to host-cell carbohydrate, specifically recognizing asialo-lactosyl-containing carbohydrates.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cormack, B P -- Ghori, N -- Falkow, S -- New York, N.Y. -- Science. 1999 Jul 23;285(5427):578-82.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, Stanford University School of Medicine, Fairchild D039, 299 Campus Drive, Stanford, CA 94305-5124, USA. bcormack@jhmi.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10417386" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Calcium/metabolism
;
Candida/*genetics/*pathogenicity/physiology
;
Candidiasis, Vulvovaginal/microbiology
;
Carbohydrates/pharmacology
;
Cell Adhesion
;
Cloning, Molecular
;
Epithelial Cells/*microbiology
;
Female
;
*Fungal Proteins
;
Genes, Fungal
;
Humans
;
Lectins/chemistry/*genetics/metabolism
;
Ligands
;
Mice
;
Mice, Inbred DBA
;
Molecular Sequence Data
;
Mutagenesis, Insertional
;
Mutation
;
Plasmids
;
Polymerase Chain Reaction
;
Transformation, Genetic
;
Tumor Cells, Cultured
;
Virulence/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
