Publication Date:
2009-03-17
Description:
As fundamental units of neuronal communication, chemical synapses are composed of presynaptic and postsynaptic specializations that form at specific locations with defined shape and size. Synaptic assembly must be tightly regulated to prevent overgrowth of the synapse size and number, but the molecular mechanisms that inhibit synapse assembly are poorly understood. We identified regulator of synaptogenesis-1 (RSY-1) as an evolutionarily conserved molecule that locally antagonized presynaptic assembly. The loss of RSY-1 in Caenorhabditis elegans led to formation of extra synapses and recruitment of excessive synaptic material to presynaptic sites. RSY-1 directly interacted with and negatively regulated SYD-2/liprin-alpha, a master assembly molecule that recruits numerous synaptic components to presynaptic sites. RSY-1 also bound and regulated SYD-1, a synaptic protein required for proper functioning of SYD-2. Thus, local inhibitory mechanisms govern synapse formation.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087376/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087376/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Patel, Maulik R -- Shen, Kang -- 1R01NS048392/NS/NINDS NIH HHS/ -- R01 NS048392/NS/NINDS NIH HHS/ -- R01 NS048392-05/NS/NINDS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2009 Mar 13;323(5920):1500-3. doi: 10.1126/science.1169025.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Neurosciences Program, Stanford University, 385 Serra Mall, Herrin Labs, Room 144, Stanford University, Stanford,CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19286562" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Animals, Genetically Modified
;
Caenorhabditis elegans/genetics/*physiology
;
Caenorhabditis elegans Proteins/chemistry/genetics/*metabolism
;
Carrier Proteins/metabolism
;
Cell Line
;
Cell Nucleus/metabolism
;
Humans
;
Mutation
;
Nerve Tissue Proteins/metabolism
;
Nuclear Proteins/chemistry/genetics/*metabolism
;
Phosphoproteins/genetics/metabolism
;
Protein Binding
;
Protein Interaction Mapping
;
Protein Isoforms/chemistry/genetics/metabolism
;
Protein Structure, Tertiary
;
Recombinant Fusion Proteins/metabolism
;
Synapses/metabolism/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
