Publication Date:
2005-12-22
Description:
We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Poinar, Hendrik N -- Schwarz, Carsten -- Qi, Ji -- Shapiro, Beth -- Macphee, Ross D E -- Buigues, Bernard -- Tikhonov, Alexei -- Huson, Daniel H -- Tomsho, Lynn P -- Auch, Alexander -- Rampp, Markus -- Miller, Webb -- Schuster, Stephan C -- HG02238/HG/NHGRI NIH HHS/ -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 2006 Jan 20;311(5759):392-4. Epub 2005 Dec 20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉McMaster Ancient DNA Center, McMaster University, 1280 Main Street West, Hamilton ON, L8S 4L9 Canada. poinarh@mcmaster.ca〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16368896" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Composition
;
Computational Biology
;
Cytochromes b/genetics
;
DNA, Mitochondrial/genetics
;
Dogs/genetics
;
Elephants/*genetics
;
*Fossils
;
Gene Library
;
Genome
;
*Genomics
;
Humans
;
Mandible/*chemistry
;
*Paleontology
;
Polymerase Chain Reaction
;
Sequence Alignment
;
*Sequence Analysis, DNA
;
Sequence Homology, Nucleic Acid
;
Siberia
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
