Publication Date:
2004-04-06
Description:
The fat-derived hormone leptin regulates energy balance in part by modulating the activity of neuropeptide Y and proopiomelanocortin neurons in the hypothalamic arcuate nucleus. To study the intrinsic activity of these neurons and their responses to leptin, we generated mice that express distinct green fluorescent proteins in these two neuronal types. Leptin-deficient (ob/ob) mice differed from wild-type mice in the numbers of excitatory and inhibitory synapses and postsynaptic currents onto neuropeptide Y and proopiomelanocortin neurons. When leptin was delivered systemically to ob/ob mice, the synaptic density rapidly normalized, an effect detectable within 6 hours, several hours before leptin's effect on food intake. These data suggest that leptin-mediated plasticity in the ob/ob hypothalamus may underlie some of the hormone's behavioral effects.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pinto, Shirly -- Roseberry, Aaron G -- Liu, Hongyan -- Diano, Sabrina -- Shanabrough, Marya -- Cai, Xiaoli -- Friedman, Jeffrey M -- Horvath, Tamas L -- DK060711/DK/NIDDK NIH HHS/ -- F32DK61176/DK/NIDDK NIH HHS/ -- F32NS046921/NS/NINDS NIH HHS/ -- R01 DK041096/DK/NIDDK NIH HHS/ -- R01 DK061619/DK/NIDDK NIH HHS/ -- RR014451/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2004 Apr 2;304(5667):110-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Molecular Genetics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15064421" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Arcuate Nucleus of Hypothalamus/cytology/*physiology
;
Body Weight/drug effects
;
Eating
;
Evoked Potentials
;
Excitatory Postsynaptic Potentials
;
*Feeding Behavior/drug effects
;
Ghrelin
;
Glutamic Acid/analysis
;
Green Fluorescent Proteins
;
In Vitro Techniques
;
Leptin/genetics/pharmacology/*physiology
;
Luminescent Proteins/analysis
;
Mice
;
Mice, Obese
;
Mice, Transgenic
;
Neuronal Plasticity/*physiology
;
Neurons/drug effects/*physiology
;
Neuropeptide Y/genetics/physiology
;
Patch-Clamp Techniques
;
Peptide Hormones/pharmacology
;
Pro-Opiomelanocortin/genetics/physiology
;
Recombinant Fusion Proteins/analysis
;
Synapses/chemistry/ultrastructure
;
Tetrodotoxin/pharmacology
;
Transgenes
;
gamma-Aminobutyric Acid/analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
