Publication Date:
2018-09-14
Description:
Algal biomass emerged as a potential source of bioenergy and valuable derivatives in recent years. The major characteristics of microalgae such as high oil contents, carbon sequestration, high growth rate and valuable by-products are leading factors to compete with traditional resources. The aim of current study was (i) to extract and optimize chlorophylls (a and b) at temperature (30-40°C) and time (60-120 min) by ultrasonication assisted with methanol: hexane (2:1v/v) (ii) to find suitable dilution factor for chlorophyll analysis by UV spectroscopy considering 1:10, 1:15,1:20 ml/ml and (iii) to determine suitability of dissolving methanol/hexane extract in different solvents for analysis and quantification by simultaneous equations. The full factorial design RSM was employed and found that model is well fitted (0.99 R 2 ). Maximum recovery of total chlorophylls (a and b) was 17.15 μg/ml achieved at 30 °C and 120 min. The absorbance spectra peaks were found good with a...
Print ISSN:
1757-8981
Electronic ISSN:
1757-899X
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics