Publication Date:
2018-09-11
Description:
by Rafael Campos, Alberto Fernando Oliveira Justo, Fabíola Z. Mónica, José Carlos Cogo, Ronilson Agnaldo Moreno, Valéria Barbosa de Souza, Andre Almeida Schenka, Gilberto De Nucci Endothelium is the main source of catecholamine release in the electrical-field stimulation (EFS)–induced aortic contractions of the non- venomous snake Panterophis guttatus . However, adrenergic vasomotor control in venomous snakes such as Crotalus durissus terrificus and Bothrops jararaca has not yet been investigated. Crotalus and Bothrops aortic rings were mounted in an organ bath system. EFS-induced aortae contractions were performed in the presence and absence of guanethidine (30 μM), phentolamine (10 μM) or tetrodotoxin (1 μM). Frequency-induced contractions were also performed in aortae with endothelium removed. Immunohistochemical localization of both tyrosine hydroxylase (TH) and S-100 protein in snake aortic rings and brains, as well as in human tissue (paraganglioma tumour) were carried out. EFS (4 to 16 Hz) induced frequency-dependent aortic contractions in both Crotalus and Bothrops . The EFS-induced contractions were significantly reduced in the presence of either guanethidine or phentolamine in both snakes (p
Electronic ISSN:
1932-6203
Topics:
Medicine
,
Natural Sciences in General
