ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
A detection, viability, and infectivity assay was developed for Cryptosporidiurn parvum. Oocysts or excysted sporozoites were inoculated onto monolayers of CaCo-2 cells grown on chamber slides. C. parvum infection was monitored by three methods: a) application of a fluorescein-labeled anti-sporozoite antibody; b) PCR of a heat-shock protein gene fragment; and c) detection of mRNA from the heat-shock protein gene by RT-PCR.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.1996.tb05003.x