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Non-axenic and Axenic Growth of Vorticella microstoma (1959)

FINLEY, HAROLD E. ; McLAUGHLIN, DAVID ; HARRISON, DORIS M.

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 6 (1959), S. 0

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ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: SYNOPSIS. Vorticella microstoma was grown non-axenically and axenically at pH 6.4. Vorticellas were maintained indefinitely on Bacillus cereus in a medium composed of Proteose-Peptone, Cerophyl, and the filtrate from boiled wheat kernels. Prolific growth occurred in 2-membered cultures. A medium containing hydrolyzed gelatin, aqueous liver extract, yeast nucleic acid hydrolysate, glucose, and penicillin is recommended for axenic growth.The potential value of vorticellids as research tools is discussed together with metabolic implications of supplementing sterile Proteose-Peptone broth with natural substances in particle form. The ineffectiveness of adding tryptophan, thiamine, glycine, and chelating agents to the axenic medium was considered. Refinements of the axenic medium are on trial.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1959.tb03952.x

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