ISSN:
1365-2761
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Abstract. The effectiveness of three different growth media KDM-2, S-KDM and S-KDM-C for primary isolation of Renibacterium salmoninarum was examined over a 14-week incubation period. Kidney samples were taken from Atlantic salmon, Salmo salar L., broodfish returning to a sea ranch after 2 years at sea. Homogenized samples were inoculated onto two selective media, S-KDM supplemented with lamb serum and S-KDM-C supplemented with activated charcoal. The third medium was the non-selective serum supplemented KDM-2. Renibacterium salmoninarum was isolated from 112 samples on one or more of the media used. Of all positive samples, 91% were positive on S-KDM, 60% on S-KDM-C and 35% on KDM-2. These results demonstrate that selectivity significantly enhances the isolation capacity of the medium and that supplementing with serum is significantly more effective than supplementing with charcoal.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2761.1991.tb00579.x
