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Characterisation of the cell envelope of Treponema hyodysenteriae (1988)

Chatfield, Steven N. ; Fernie, David S. ; Beesley, Julian ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 55 (1988), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract Cell envelope preparations of Treponema hyodysenteriae (strain CN 8368) were examined using biochemical and immunochemical methods. Several major polypeptides were detected with molecular weight between 24-kDa and 45-kDa. The majority of these polypeptides were recognised by serum from a pig vaccinated with an experimental whole-cell T. hyodysenteriae vaccine and hyperimmune anti-T. hyodysenteriae rabbit sera. Immune electron microscopy confirmed that the major antigens detected were associated with the cell envelope. Triton X-100, in the presence of EDTA, completely solubilised a polypeptide with an approximate molecular weight of 36-kDa. Antibodies to this polypeptide were not absorbed by whole T. innocens cells.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02819.x

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