ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Leaching bacteria attach to their substrates, i.e. mineral sulfides, and form monolayeredbiofilms. In this study the biofilm formation of Acidithiobacillus ferrooxidans A2 on pyrite wasexamined using atomic force and epifluorescence microscopy (AFM and EFM, respectively). Anovel system by JPK instruments, the BioMaterial WorkstationTM, allows the investigation of thesame location on an opaque sample with AFM and EFM. Until recently this was only possible fortranslucent samples. Sessile bacteria on pyrite coupons were stained with 4’,6-diamidino-2-phenylindol (DAPI) and visualized by EFM as well as AFM. The best imaging conditions for AFMwere assessed. Scans of bacteria attached to pyrite were performed in contact mode in air as well asin tapping mode in fluid. Imaging in fluid was more challenging than imaging in air as bacteria tendto detach from their substratum. To avoid the dislocation of microorganisms by the AFM probe thesample was dried in air for 1 h prior to scanning in fluid. Scanning in air was performed with thewhole range of cantilever spring constants tested (k = 0.03 N/m to k = 0.65 N/m) while, forscanning in fluid, best results were achieved using stiffer cantilevers (k = 0.65 N/m)
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.371.pdf
