ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Reversed phase operation
;
Calculation of optimum solvent composition
;
Polymethoxylated flavones
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary An optimization procedure for the chromatographic separation of polymethoxylated flavones is described. A polyhedron working volume is drawn from a truncated tetrahedron constructed from the four solvents selected. The response function is an n'th degree polynomial the coefficients of which are calculated through the least squares method by means of B=(X′X)−1X′Y in which X′X is the information matrix (X′X)−1 the variance covariance matrix. X is the matrix of the model (polynomial built from the selected experiments) and X′ the transposed matrix. Y is the quality of the chromatogram defined by the resolution of each pair of solutes. An exchange algorithm matrix yields the minimum number of runs necessary to obtain a correct B. Isoresponses curves are drawn by intersection of a cubic volume which includes the polyhedron to give a better insight. An optimal zone is then determined and provides the solvent composition. An optimized chromatogram of 8 methoxylated flavones demonstrates the application of the method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02310411
