ISSN:
1612-1112
Keywords:
Thin-layer chromatography
;
Optimum separation
;
Binary mobile phase mixtures
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Up to now the optimum composition of a binary mobile phase for separation in thin-layer chromatography has been determined mainly by the method of successive trials. In this paper a simple way is presented to determine the mobile phase composition and the maximum difference in the RM values of two substances. The equation introduced has been illustrated with examples of adsorption thin-layer chromatography on magnesium silicate. Calculation of the largest difference in the RM values of two substances in relation to the composition of the binary mobile phase has been compared with the corresponding values determined experimentally. A good agreement of the obtained theoretical data and experimental values has been found.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02261874
