ISSN:
1615-6102
Keywords:
Immunocytochemistry
;
Non-articulated laticifers
;
Articulated laticifers
;
Asclepiadaceae
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary sensitive immunocytochemical method for the identification of laticifers has been developed. Frozen sections of various laticifer-bearing plant material, mounted on slides, were first flooded with the IgG fraction of rabbit anti-latex antiserum, prepared using whole latex ofAsclepias syriaca, then flooded with fluorescein-conjugated IgG fraction goat anti-rabbit IgG to visualize laticifers. Positive fluorescence was observed for laticifers in shoots and embryos ofA. syriaca andStapelia bella and embryos ofA. tuberosa. Laticifers did not fluoresce in shoots ofA. tuberosa andEuphorbia tirucalli, in embryos ofE. marginata, or in petioles ofMusa paradisiaca andCichorium intybus. Controls prepared with uninjected rabbit serum were negative (no fluorescence).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01279440
