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Identification of a male-specific (H-Y) antigen on the flagellar plasma membrane of ram epididymal spermatozoa (1987)

Bradley, Mark P. ; Forrester, Ian T. ; Heslop, Barbara F.

Springer

Human genetics 〈Berlin〉 75 (1987), S. 362-367

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ISSN: 1432-1203

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary H-Y (male-specific) antigen has been detected on the plasma membranes of both caput and caudal ram spermatozoa using both immunoperoxidase and immunofluorescence labelling techniques. In these spermatozoa the distribution of H-Y antigen appears to be confined to both the posterior region of the head and the mid-piece region of the flagellum. In addition, caput spermatozoa also exhibit intense immunoperoxidase staining of the cytoplasmic droplet which is situated on the flagellum at the base of the head. Western blot analyses of purified plasma membranes from the flagella of caudal spermatozoa have revealed the presence of a malespecific protein with an estimated molecular weight of 25,000–27,000.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00284109

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