ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The UV enhancement of recombination among λ bacteriophages was studied in host bacteria that are defective in DNA repair and compared to that observed in wild type host strains. The following conclusions were drawn: 1. The UV enhancement of recombination is even greater in HCR− than in HCR+ host strains. The UV lesions that promote recombination are repaired by host cell reactivation with the same efficiency as are potentially lethal UV lesions. 2. The UV enhancement of λ recombination is the same in HCR+ host strains that are deficient in their own recombination as it is in the wild type hosts. 3. Very few lesions per phage particle are sufficient to stimulate the enhanced recombination. The UV-stimulated recombination occurs during the same time interval as host cell reactivation. 4. There is substantial multiplicity reactivation of UV lesions in λ. Similar numbers of recombination events neutralize similar numbers of UV lesions in HCR+ and HCR− hosts, but a much greater survival increase is observed in the latter.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00333603
