ISSN:
1617-4623
Keywords:
Heat shock regulatory element
;
Pathogenesis-related proteins (PR-proteins)
;
Plant gene sequence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Pathogenesis-related proteins (PR-proteins) are a heterogeneous group of host encoded, low molecular weight proteins, which are induced in plants by different external stimuli such as pathogen attack or exposure of the plants to certain chemicals. Three independent bacteriophage λ clones hybridizing to PR-1 cDNA probes were isolated from a tobacco (Nicotiana tabacum cv. Wisconsin 38) genomic library. All three clones are derived from different genomic locations and contain single gene copies. DNA sequence analysis revealed that one of the genomic clones is essentially identical to the PR-1a cDNA clones described previously from tobacco mosaic virus infected tobacco plants (Nicotiana tabacum cv. Samsun NN). This clone contains 335 bp of the 5′ upstream and the entire transcribed regions. The PR-1a gene is not interrupted by intervening sequences. In the 5′ upstream region, characteristic eukaryotic transcription signals as well as putative regulatory sequences were identified. A consensus TATA box is found at position-34 and an-11 pb long imperfect direct repeat is present at position-116 and-135 with respect to the transcription initiation site. Furthermore, the sequence C-GAA-TTC-G, which differs from the consensus heat shock regulatory element only by the insertion of one extra nucleotide, is located at position-57. The presence of this element suggests a molecular relationship of heat shock and pathogen responses in plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330606
