ISSN:
1617-4623
Keywords:
Wheat
;
Photosystem II
;
Cytochrome b-559
;
Chloroplast genes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The gene for the apoprotein of cytochrome b-559 has been located in wheat chloroplast DNA by coupled transcription-translation of fragments of chloroplast DNA in a cell-free system from Escherichia coli and by nucleotide sequence analysis. The gene is located 1.5 kbp downstream from the gene for cytochrome f and is transcribed in the opposite direction. Nucleotide sequence determination revealed an open reading frame coding for a hydrophobic protein of 83 amino acid residues (M r 9434). The deduced amino acid sequence of the first 27 codons corresponds exactly to the determined N-terminal amino acid sequence of spinach cytochrome b-559. Another open reading frame of 39 codons is located 10 bp downstream from the gene for cytochrome b-559. A putative ribosome-binding site, AGGAGG, for this open reading frame overlaps the translation stop codon, UAG, of the cytochrome b-559 gene. The deduced amino acid sequence from this open reading frame shows similarities to the N-terminal sequence of cytochrome b-559. Both sequences contain a histidine residue located in a putative membrane-spanning region of the polypeptide.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330389
