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Mu-1 directed inhibition of DNA breakdown in Escherichia coli, recA cells (1978)

Vliet, F. ; Couturier, M. ; Lafonteyne, J. ; [et al.]

Springer

Molecular genetics and genomics 164 (1978), S. 109-112

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ISSN: 1617-4623

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The spontaneous DNA breakdown exhibited by recA strains, is reduced after heat induction of a thermoinducible Mu-1 prophage. This inhibition is dependent upon RNA synthesis, suggesting that Mu-1 directs synthesis of a recBC nuclease inhibitor, analogous to the product of the λ gam gene. The genetic evidence presented here shows that Mu-1 enables a λ red gam phage to grow on a recA host. The in vitro assay for ATP-dependent exonuclease activity reveals a complete inhibition of this activity 30 min after induction of the Mu-1 prophage.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00267606

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