Electronic Resource
Springer
Molecular genetics and genomics
159 (1978), S. 151-160
ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary An approach for the screening of mit - mutants, the isolation and preliminary classification of a series of such mutants is reported. Loss and retention of 8 mit - and 6 drug r markers in mitDNA was analyzed in populations of rho- clones derived from four yeast strains. The populations studied constitute a representative fraction of the rho- petites formed during growth at 35° C under the influence of mutation tsp-25 which is in common to the four strains. The majority of the rho- clones retained several of the markers studied. Depending on the marker regarded retention frequencies between 15% (oxi3) and 45% (oli1, cob) were observed. Loss of one and retention of the other of a pair of markers was determined in all rho- clones of the four populations. The frequencies of marker separation by rho- deletion thus obtained are assumed to reflect the distance between markers on the mitochondrial genome: the higher the frequency of separation the longer the distance between two markers. Based on these frequencies a unique order of markers on a circular map was determined. Positions of markers on a scale from 0 to 100 were found to be: cap/ery (0) — olil (16) — cob1-1354 (21) — ana101 (22) — cob2-1625 (24) — oli2 (35) — pho1 (40) — oxi3-2501 (44) — oxi3-3771 (47) — par (65) — oxi2 (79) — oxil (87) tms8 (93) —cap (100). The relevance of this map as to the faithful representation of the topology of gene loci on mitDNA is discussed. Correlation of retention frequencies of markers to their map positions reveals a pronounced polarity: mitDNA segments carrying the cob-oli1 segment prevail whereas segments retaining oxi3 are the least frequent.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00270888
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