Electronic Resource
Springer
Molecular genetics and genomics
110 (1971), S. 327-331
ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Pre-competent and competent cultures of Bacillus subtilis were x-irradiated before and after centrifugal separation of cells in a Renografin density gradient. Pre-competent cultures have no cells at the radiosensitivity of the cells in the bulk competent culture, but there is a substantial fraction of cells in a multi-target state, with heterogeneous target numbers. On reaching maximal competence, the survival becomes entirely linear in radiosensitivity. Irradiation of the separated competent cells shows that competence development correlates with disappearance of multi-target cells from the non-competent band of cells and the appearance of single-target cells in the competent band at a radiosensitivity equal to that of the bulk competent culture. Thus the multi-target state may be a required stage in the development of competence in this system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00438274
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