ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A technique for demonstration of glycosaminoglycans and other biological polyanions has been applied on floating thin sections for electron microscopy. The method is based on the formation of insoluble polyanions-quaternary ammonium salt complexes and the subsequent formation of electron dense ion association complexes following a ferric thiocyanate treatment. Mast cell granules and eosinophilic granule crystalloids were selectively stained. Nucleic acid contrast was selective to some extends.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00533264