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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 13 (1968), S. 7-10 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary There is some confusion in the literature as to whether the protein carboxyl groups demonstrable with Barrnett and Seligman's histochemical technique are C-terminal or side-chain. It seems that in the first step of this technique, hot mixtures of acetic anhydride and pyridine convert the commonly-occurring side-chain carboxyls into mixed acid anhydrides. That they do has now been confirmed; proteins in tissue sections treated with such acetic anhydride mixtures do not react with hydroxynaphthoic acid hydrazide after being left for a long time in either cold methanol or a cold solution of aniline in xylene. In this they resemble the acid anhydrides used for synthezising peptides in vitro. Thus all the lilac-reddish colours observable in tissue proteins on which the Barrnett and Seligman technique proper has been carried out can be ascribed to side-chain carboxyl groups.
    Type of Medium: Electronic Resource
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