ISSN:
1432-0886
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract In this study a technique is used which allows observation of chromatin fibers in sections of isolated liver nuclei of the rat. The fibers, which exhibit a rather uniform diameter (diameter = 125 ± 20 Å) seem to be constructed of coiled or folded sub-filaments. — After application of a preferential ribonucleoproteinstain (EDTA-method) only parts of the fibers retain their stain whereas others are completely bleached. Finer filaments (diameter = 45 Å) seem to be continuous with the stained parts of the chromatin fibers. The RNP-stain is lost when the nuclei have been treated with RNase. From these observations it is concluded that RNA-rich and RNA-free segments exist on chromatin fibers which may be related to RNA transcription processes. — Granules and thin filaments are the only structures stained with the EDTA-method in the nucleolus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331801
