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  • 1
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new optical fiber sensor was prepared for the determination of berberine in aqueous solution using a micrometer-sized flow-cell and a bifurcated optical fiber. The sensing is based on fluorescence quenching of 2-(4-diphenylyl)-6-phenylbenzoxazole (PBBO) in the PVC membrane. This process is accompanied by non-fluorescent ground-state complex formation. With this sensor, berberine can be determined in sample solutions from 2.42 × 10–5 mol L–1 to 6.04 × 10–7 mol L–1. Satisfactory reproducibility, reversibility, and short response times of less than 1 min are realized. The sensor also shows good selectivity over some common pharmaceutical species and alkali and alkali-earth metal salts, and can be used for the direct assay of berberine in commercial tablets. The results are in correspondence with those obtained by the pharmacopoeia method.
    Type of Medium: Electronic Resource
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