Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Abstract Horseradish peroxidase (HRP) was effectively entrapped in a novel composite membrane of poly-vinyl alcohol and regenerated silk fibroin, and IR was employed to provide a useful insight into the structure of the composite membrane. A methylene blue-mediated sensor highly sensitive to hydrogen peroxide was constructed, which was based on the immobilization of HRP in the composite membrane. Cyclic voltammetry and amperometric measurement were utilized to demonstrate the feasibility of electron communication between immobilized HRP and a glassy carbon electrode in the bioelectrocatalytic reduction of hydrogen peroxide via methylene blue. Performance and characteristics of the sensor were evaluated with regard to response time, detection limit, selectivity, and dependence on temperature and pH as well as operating and storage stability. The sensor possesses a variety of characteristics including high sensitivity, rapid response time and a low detection limit of 0.1 μmol/L.
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