ISSN:
1573-904X
Keywords:
ethanol
;
diffusion enhancer
;
metabolism inhibitor
;
β-estradiol
;
hairless mouse skin
;
quantitative biophysical model
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The influence of low levels of ethanol on the simultaneous diffusion and metabolism of β-estradiol (E2β) in hairless mouse skin was quantitatively evaluated. A wide range of diffusion/metabolism experiments was conducted with full-thickness skin, stripped skin, and dermis at the various ethanol levels. The experiments were carried out in a two-chamber diffusion-cell system where ethanol was present in both the donor and the receiver chambers at equal concentrations. Analysis of the experimental data with several enzyme distribution models further showed that the best model was that for which the enzyme activity resided totally in the epidermis and near the basal layer of the epidermis. The ethanol effects were separated and quantified in terms of the diffusion and metabolism parameters. Aqueous ethanol, even at low concentrations (≤25%), was found to have two important effects on E2β transport: ethanol functions as an inhibitor of the enzymatic conversion of E2β to estrone (E1) in the viable epidermis, and ethanol is able to enhance the transport of permeants across the lipoidal pathway of the stratum corneum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1015847311266
