ISSN:
1423-0127
Keywords:
Thrombin
;
Thapsigargin
;
Inositol 1,4,5-trisphosphate
;
Cation channel
;
nonselective
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Ca2+ influx is an important event associated with platelet activation and regulated by the content of intracellular Ca2+. Previous studies have suggested two different Ca2+ pools and two Ca2+ influx pathways exist in platelets. In the present study, we have investigated the regulation of thrombin- and thapsigargin-induced Ca2+ entry into human platelets, using fluorescent indicators to monitor Ca2+ mobilization and membrane potential. It was found that depletion of thapsigargin-sensitive Ca2+ stores was coupled to Ca2+ influx through a Ca2+-selective pathway. Additional release of Ca2+ from the thapsigargin-insensitive pool by thrombin caused the opening of a nonselective cation channel.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02255916
