ISSN:
1573-5044
Keywords:
BAP
;
elongation phase
;
NAA
;
micropropagation
;
physiological state
;
rooting, shoot multiplication
;
tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Populus x wilsocarpa, a hybrid of important ornamental value, cannot be seed-propagated, nor grafted, since a compatible rootstock has not been identified. A micropropagation protocol consisting of a series of steps was therefore developed to facilitate the commercial production of this species. The technique involved the transfer of swelling buds to a growth initiation medium with the following composition: N6 macronutrients, MS micronutrients and vitamins supplemented with 0.5 mg l-1 BAP. The best buds were from dormant twigs, stored at 0–2°C and then forced to burst prior to culture initiation. Shoot multiplication was on a basal WPM medium including 0.1 mg l-1 BAP and 0.001 mg l-1 NAA. Shoot elongation and rooting was also on a basal WPM medium supplemented with 1.0 mg l-1 GA3 followed by a transfer to a peat-perlite mix in the greenhouse.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00047747
