ISSN:
1573-5028
Keywords:
maize (Zea mays)
;
chloroplast operon
;
chloroplast transcription
;
ribosomal protein genes
;
thylakoid ATP synthase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Several examples of the introduction of a gene from one gene complex into another (introgression) are found when chloroplast RP gene clusters are compared to those in Escherichia coli or cyanobacteria. Here we describe the transcript pattern of one such cluster from maize (Zea mays) that includes the genes for 4 subunits of the thylakoid ATP synthase (atpI, H, F, A) and the rps2 gene. Twelve transcript species covering the size range from 7 000 to 800 nt were identified in RNA isolated from dark-grown and greening maize seedlings, and several of them were characterized by reverse transcription analysis. A major species of 6 200 nt, with its 5′ end at 181 nt upstream of the initiating ATG of rps2, contained the transcripts of all the 5 genes. Two further sets of transcripts having their 5′ ends ca. 120 and 50 nt upstream of the initiation codons of the atpI and atpH genes were also identified. Thus, this plastid gene cluster in maize is functionally organized as an operon with additional regulatory features to allow for increased accumulation of mRNAs for the thylakoid components.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00023603
