ISSN:
1573-5028
Keywords:
electron transfer
;
light-harvesting complex I
;
membrane localization
;
photosynthesis
;
processing site
;
transit peptide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We report here the isolation and nucleotide sequence of a cDNA clone encoding a phtosystem I polypeptide that is recognized by a polyclonal antibody prepared against subunit II of the photosystem I reaction center. The transit peptide processing site was determined to occur after Met50 by N terminal sequencing. The decuced sequence of this protein predicts that the polypeptide has a net positive charge (pI=9.6) and no membrane spanning regions are evident from the hydropathy plot. Based on these considerations and the fact that subunit II is solubilized by alkali treatment of thylakoids, we concluded that subunit II is an extrinsic membrane protein. The absence of hydrophobic regions characteristic of thylakoid transfer domains furthermore implies that subunit II is localized on the stromal side of the membrane.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00014949
