ISSN:
0362-2525
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Biochemical assay of acid phosphatase in normal and lens-regenerating eyes of the urodele Diemictylus viridescens, using p-nitrophenyl phosphate as substrate, demonstrates both soluble and lysosomal fractions of the enzyme. While the specific activity of the soluble fraction remains unchanged during lens regeneration, the lysosomal fraction shows four distinct rises in specific activity during the thirty-day regeneration period studied. These peak activities on the second, eighth, fifteenth, and twenty-second days post-lentectomy apparently correspond to lysosomal activity in the processes of wound healing, iris depigmentation, and lens differentiation which occur during urodele lens regeneration. On the basis of biochemical and histochemical studies as well as observations of morphological changes in the urodele eye as lens regeneration proceeds, it is postulated that there is a significant correlation between these morphological changes and the level and localization of the lysosomal acid hydrolases in the tissues in which the changes occur.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jmor.1051290307