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Localization of a protein a-tagged kex2 protein to the vacuole of Saccharomyces cerevisiae allows rapid purification of vacuolar membranes (1995)

Bryant, Nia J. ; Boyd, Alan

New York, NY [u.a.] : Wiley-Blackwell

Yeast 11 (1995), S. 201-210

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ISSN: 0749-503X

Keywords: Saccharomyces cerevisiae ; protein trafficking ; vacuole ; cell fractionation ; Life and Medical Sciences ; Genetics

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: We have previously reported an immunoisolation procedure which allows purification of Kex2p-containing Golgi membranes from lysed yeast cells. In order to evaluate the use of tagging procedures in organelle isolation we set out to isolate the same Golgi membrane fraction using a version of the Kex2 protease that had been affinity-tagged at its C-terminus. This protein is found to be localized in the vacuole, providing the basis of a method for the affinity-purification of vacuolar membranes.

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/yea.320110302

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