ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The present paper gives a description of a procedure for isolation of pepsinogen I from human urine. The initial steps are concentration by batch adsorption to DEAE-Sephadex, desorption by addition of NaCl, and DEAE-chromatography. The final step is preparative isotachophoresis with Ampholine carrier ampholytes as spacers. Exact visualization of the fractionations is obtained by fused rocket immunoelectrophoresis. The procedure provides pepsinogen I of high purity as shown by crossed immunoelectrophoresis, agar gel electrophoresis and N-terminal aminoacid analysis. The yield of the procedure is 0.05-0.1 mg pepsinogen I per liter urine.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150010308
