ISSN:
1040-0397
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A flow-injection system is proposed for the estimation offish freshness. The 5′-adenylic acid deaminase reactor, alkaline phosphatase reactor, and nucleoside phosphorylase-xanthine oxidase coimmobilized reactor were incorporated at fixed positions in a flow system, which was based on the splitting of the flow after sample injection and subsequent confluence before reaching the peroxidase electrode. Because each channel has a different residence time, two peaks were obtained. The first peak corresponded to the total of hypoxanthine and inosine, and the second peak to the total of hypoxanthine, inosine, inosine-5′-monophosphate, and adenosine-5′-monophosphate. The index of fish freshness, K, is estimated by \documentclass{article}\pagestyle{empty}\begin{document}$$ K = (S_2 /S_1)(i_1 /i_2)\,{\rm x}\,{\rm 100} $$\end{document} where s1 and s2 represent the sensitivity (nA mM-1) and i1 and i2 represent the peak current (nA) of the first and second peaks, respectively. The measurements could be performed at a rate of 15 samples per hour with satisfactory precision.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140010214
