ISSN:
0935-6304
Keywords:
Capillary gas chromatography, HRGC
;
FTIR, GC-FTIR
;
Disaccharides sequence
;
Derivatization
;
Carbohydrates, methanolysis
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Conversion of disaccharide reducing units into the corresponding methoximes followed by their reduction with NaBH4CN to the corresponding deoxy(methoxyamino)alditol glycosides (RCH2-NHOCH3) introduces a constitutional asymmetry in the sugar molecule. After methylation of the resulting compounds, cleavage of the glycosidic bonds is carried out by BF3- catalyzed methanolysis, affording permethylated O-methylglycosides and permethylated deoxy(methylmethoxyamino)alditols with a free -OH corresponding to the position of the glycosidic bond. Identification of the reducing moiety is achieved by GC-FTIR and computerized retrieval of library FTIR spectra.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240120706