Thin-layer chromatography, TLC
TLC with mixed mobile phases
Analytical Chemistry and Spectroscopy
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
Considerations of TLC process optimization have been based on the thermodynamic theory of adsorption from multicomponent solvents using experimental and theoretical RM1, 2 = f (Φ1) relationships. It was found that a relationship exists between the Az parameter (log k1,2∞ where k1,2∞ is the partition coefficient of the substance chromatographed) of the above theory and pKa values of substances as well as the solubility parameter δ of the mobile phase components. Analysis of the Az values of substances shows that a slight variation therein is associated with lower selectivity of chromatographic separation.
Type of Medium: