Publication Date:
2012-11-16
Description:
Abstract 2509 Mantle cell lymphoma (MCL) is a specific subtype of non-Hodgkin lymphoma with a median overall survival of approximately 4–5 years with significant heterogeneity. MCL shows a relatively high number of genomic alterations per case and one of the known causes of genomic instability in somatic cells is telomere dysfunction. Short, dysfunctional telomeres lead to an increase in chromosomal end-to-end fusions which predispose the cells to chromosomal aberrations, genomic complexity and malignant transformation. Here we investigated telomere length in MCL to evaluate associations with biological and clinical disease characteristics as well as outcome. We included a CLL cohort as a control group and for comparison with MCL. A well characterized cohort of 73 MCL and 55 CLL patients treated at the Universities of Ulm, Würzburg and Heidelberg was used for the study. Telomere length was measured using a Q-PCR-based technique with 25ng of DNA used per reaction in triplicates. Three telomere length controls were included in every plate to control for plate to plate variations. The technique was validated by terminal restriction fragment length (TRF) analysis (R2=0.8516). The TRF value of telomere length for each sample was calculated from the linear regression of Q-PCR vs. TRF. Samples were selected for tumor loads of 〉70% and were analyzed for correlation with biological and clinical factors. Telomere length in MCL had no significant association with IGHV (P=0.4966) and TP53(P=0.4225) mutation status which was in marked contrast to CLL where a significant correlation of short telomeres with unmutated IGHV(Pmedian) and short (
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
