Publication Date:
2008-11-16
Description:
Object In order to compare the influences of wortmannin on platelet aggregation and platelet membrane surface glycoproteins GPIb expression after thrombin receptors activation, then to investigate the role of phosphatidylinositol 3-kinase (PI3-K) and myosin light chain kinase (MLCK) in the course of thrombin receptors activation. Methods Peptide SFLLRN (PAR1-AP) and AYPGKF (PAR4-AP)were used for stimulating platelet, then the alterations of platelet aggregation and GPIb were analyzed in the involvement of 100nM wortmannin (inhibitor of PI3-K) and 10μM wortmannin (inhibitor of MLCK). Results Platelet activation was influenced by either concentration of wortmannin in response to PARs stimulation. Platelet aggregation was apparently inhibited by 10μM wortmannin following both peptides, and slightly inhibited by 100nM wortmannin only upon PAR1-AP activation. In addition, GPIbα interalisation is partly inhibited by 100nM wortmannin in response to PAR1 (P
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine