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  • 1
    Publikationsdatum: 2012-10-05
    Beschreibung: Using fluorescence spectrometry to map autofluorescence of chlorophyll (Chl) and tryptophan (Trp) versus depth in polar ice cores in the US National Ice Core Laboratory, we found that the Chl and Trp concentrations often showed an annual modulation of up to 25%, with peaks at depths corresponding to local summers. Using epifluorescence microscopy (EFM) and flow cytometry (FCM) triggered on red fluorescence at 670 nm to study microbes from unstained melts of the polar ice, we inferred that picocyanobacteria may have been responsible for the red fluorescence in the cores. Micron-size bacteria in all ice melts from Arctic and Antarctic sites showed FCM patterns of scattering and of red vs. orange fluorescence (interpreted as due to Chl vs. phycoerythrin (PE)) that bore similarities to patterns of cultures of unstained picocyanobacteria Prochlorococcus and Synechococcus. Concentrations in ice from all sites were low, but measurable at ~ 1 to ~ 103 cells cm−3. Calibrations showed that FCM patterns of mineral grains and volcanic ash could be distinguished from microbes with high efficiency by triggering on scattering instead of by red fluorescence. Average Chl and PE autofluorescence intensities showed no decrease per cell with time during up to 150 000 yr of storage in glacial ice. Taking into account the annual modulation of ~ 25% and seasonal changes of ocean temperatures and winds, we suggest that picocyanobacteria are wind-transported year-round from warmer ocean waters onto polar ice. Ice cores offer the opportunity to study evolution of marine microbes over ~ 300 million generations by analysing their genomes vs. depth in glacial ice over the last 700 000 yr as frozen proxies for changes in their genomes in oceans.
    Print ISSN: 1726-4170
    Digitale ISSN: 1726-4189
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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