Publication Date:
1997-06-13
Description:
In allogeneic bone marrow transplantation (allo-BMT), donor lymphocytes play a central therapeutic role in both graft-versus-leukemia (GvL) and immune reconstitution. However, their use is limited by the risk of severe graft-versus-host disease (GvHD). Eight patients who relapsed or developed Epstein-Barr virus-induced lymphoma after T cell-depleted BMT were then treated with donor lymphocytes transduced with the herpes simplex virus thymidine kinase (HSV-TK) suicide gene. The transduced lymphocytes survived for up to 12 months, resulting in antitumor activity in five patients. Three patients developed GvHD, which could be effectively controlled by ganciclovir-induced elimination of the transduced cells. These data show that genetic manipulation of donor lymphocytes may increase the efficacy and safety of allo-BMT and expand its application to a larger number of patients.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bonini, C -- Ferrari, G -- Verzeletti, S -- Servida, P -- Zappone, E -- Ruggieri, L -- Ponzoni, M -- Rossini, S -- Mavilio, F -- Traversari, C -- Bordignon, C -- TGT06S01/Telethon/Italy -- TGT95000/Telethon/Italy -- New York, N.Y. -- Science. 1997 Jun 13;276(5319):1719-24.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Telethon Institute for Gene Therapy (TIGET), Istituto Scientifico H. S. Raffaele, 20132 Milan, Italy.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9180086" target="_blank"〉PubMed〈/a〉
Keywords:
*Bone Marrow Transplantation/adverse effects
;
Ganciclovir/therapeutic use
;
Gene Transfer Techniques
;
*Genetic Therapy
;
Graft vs Host Disease/etiology/*therapy
;
Humans
;
Leukemia/immunology/*therapy
;
*Lymphocyte Transfusion
;
Lymphocytes/enzymology
;
Lymphoma, Non-Hodgkin/therapy
;
Lymphoproliferative Disorders/therapy
;
Pilot Projects
;
Simplexvirus/enzymology/genetics
;
Thymidine Kinase/*genetics
;
Transplantation, Homologous
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
