Publication Date:
1997-01-24
Description:
Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed by photodamage, scattering, and indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along with measurements of the serotonin concentration ( approximately 50 mM) and content (up to approximately 5 x 10(8) molecules) of individual secretory granules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Maiti, S -- Shear, J B -- Williams, R M -- Zipfel, W R -- Webb, W W -- RR04224/RR/NCRR NIH HHS/ -- RR07719/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 1997 Jan 24;275(5299):530-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8999797" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Survival
;
Cytoplasmic Granules/*chemistry
;
Dopamine/analysis
;
Lasers
;
Microscopy, Fluorescence/instrumentation/*methods
;
Photochemistry
;
*Photons
;
Rats
;
Serotonin/*analysis
;
Tryptophan/analysis
;
Tumor Cells, Cultured
;
Ultraviolet Rays
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics