Publication Date:
1997-01-03
Description:
Chemical separations of many biomolecules and pharmaceuticals are limited by their electrostatic interaction with the surfaces of the separation medium. Mixed self-assembled monolayers of octadecyl and methyl chains organize into a dense, two-dimensionally cross-linked network over the chromatographic silica surface to reduce acid dissociation of the surface silanols. Molecular models predict that two-dimensional cross-linking is sterically possible for pure methylsiloxane monolayers, silicon-29 nuclear magnetic resonance measurements show that cross-linking predominates for mixed monolayers of primarily methylsiloxane, and chromatographic measurements confirm that electrostatic interactions are reduced when the monolayer is primarily methylsiloxane. Chromatographic separation of genetic variants of a highly charged protein, cytochrome c, demonstrates the promise of self-assembled monolayers in separations of biomolecules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wirth, M J -- Fairbank, R W -- Fatunmbi, H O -- New York, N.Y. -- Science. 1997 Jan 3;275(5296):44-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8974384" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Chromatography/*methods
;
Cytochrome c Group/isolation & purification
;
Electrochemistry
;
Hydrogen-Ion Concentration
;
Magnetic Resonance Spectroscopy
;
Models, Molecular
;
Proteins/*isolation & purification
;
Silica Gel
;
Silicon Dioxide
;
*Siloxanes/chemistry
;
Surface Properties
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
